Polymerase Chain Reaction (PCR) is a fundamental technique in molecular biology, and ensuring a contamination-free environment is crucial for accurate results. The heart of this contamination control is the PCR cabinet. In this article, we will explore the essential factors to consider when selectin
PCR: Specimen preparation areaThe main operations in this area are the preservation of clinical specimens, the extraction and storage of nucleic acids (RNA and DNA).
PCR: Strict division of work area(1) each experimental area is reasonably set;(2) each experimental area shall be clearly marked to avoid confusion of equipment, articles, reagents, etc.
Plane layout of PCR LaboratoryIn principle, the clinical gene amplification laboratory is divided into four separate working areas: reagent storage and preparation area, sample preparation area, amplification reaction mixture preparation and amplification area.
PCR work area and equipment requirements(I) reagent storage and preparation area1, 2-8c and - 15C refrigerators2. Mixer3. Micro sampler (covering 1-1000ul)4. Mobile UV lamp (near the workbench).
PCR main equipmentThe instruments and equipment in the reagent preparation area mainly include refrigerator, super clean table, sample dispenser, mixer, balance and centrifuge.
The PCR laboratory is divided into four areas. Entry into each working area must be strictly in a single direction. Different working areas use different working clothes (for example, different colors).
Polymerase Chain Reaction (PCR) is a fundamental technique in molecular biology, and ensuring a contamination-free environment is crucial for accurate results. The heart of this contamination control is the PCR cabinet. In this article, we will explore the essential factors to consider when selectin
PCR: Specimen preparation areaThe main operations in this area are the preservation of clinical specimens, the extraction and storage of nucleic acids (RNA and DNA).
PCR: Strict division of work area(1) each experimental area is reasonably set;(2) each experimental area shall be clearly marked to avoid confusion of equipment, articles, reagents, etc.
Plane layout of PCR LaboratoryIn principle, the clinical gene amplification laboratory is divided into four separate working areas: reagent storage and preparation area, sample preparation area, amplification reaction mixture preparation and amplification area.
PCR work area and equipment requirements(I) reagent storage and preparation area1, 2-8c and - 15C refrigerators2. Mixer3. Micro sampler (covering 1-1000ul)4. Mobile UV lamp (near the workbench).