Publish Time: 2022-09-13 Origin: Site
(I) reagent storage and preparation area
1, 2-8c and - 15C refrigerators
2. Mixer
3. Micro sampler (covering 1-1000ul)
4. Mobile UV lamp (near the workbench)
5. Consumables: disposable gloves, disposable absorbent paper, high-pressure resistant centrifuge tube and sampler tip (with filter core)
6. Special work clothes and shoes
7. Special office supplies
(II) specimen preparation area
1. 2-8c refrigerator, - 20C or - 80C refrigerator
2. High speed desktop refrigerated centrifuge
3. Mixer
4. Water bath box or heating module
5. Micro sampler (covering 1-1000ul)
6. Movable UV lamp (near the working table)
7. Biosafety cabinet
8. Consumables: disposable gloves, disposable absorbent paper, high-pressure resistant centrifuge tube and sampler tip (with filter core)
9. Special work clothes and shoes
10. Special office supplies
If macromolecular DNA needs to be processed, it should have an ultrasonic water bath.
(III) preparation of amplification reaction mixture and amplification area
1. Nucleic acid amplifier
2. Micro sampler (covering 1-1000ul)
3. Movable UV lamp (near the working table)
4. Consumables: disposable gloves, disposable absorbent paper, high-pressure resistant centrifuge tube and sampler tip (with filter core)
5. Special work clothes and shoes
6. Special office supplies
(IV) amplification product analysis area
Depending on the inspection method. Basic instruments and equipment are as follows:
1. Micro sampler (covering 1-1000ul)
2. Movable UV lamp (near the workbench)
3. Consumables: disposable gloves, disposable absorbent paper, sample feeder tip (with filter core)
4. Special work clothes and shoes
5. Special office supplies
5. The safety of personnel and samples is jointly guaranteed by the biosafety cabinet and the directional airflow. The PCR laboratory design procedure stipulates that personnel and samples must flow in one direction. That is, "from the reagent preparation area to the sample preparation area, and then from the sample preparation to the amplification area and the product analysis area", the reverse flow is absolutely not allowed to avoid cross contamination. The gas flow direction only specifies that the reagent preparation area is micro positive pressure or normal pressure, the sample preparation area is normal pressure or negative pressure, the amplification area and the product analysis area are negative pressure, and the pressure gradient is from reagent preparation to product analysis area, which is high to low. The regulations do not specify that a fresh air system should be made, but in order to reduce the risk of cross pollution, it is recommended to make a fresh air system.
1 Overview
Clinical gene amplification experiment, also known as PCR experiment, is a detection method specially used to detect viral infectious diseases such as AIDS, hepatitis B and avian diseases. It can detect whether some infected people with low virus content contain specific viruses by amplifying the genes contained in the virus. Because this detection method can detect viruses that are difficult to be detected by ordinary tests and has the advantages of high sensitivity, high specificity, rapidity and low sample requirements, it is widely recognized by clinicians and has been widely used in clinical diagnosis of hospitals and diagnosis of avian diseases in epidemic prevention detection departments. However, this kind of experiment requires a laboratory that can ensure absolute safety, reasonable configuration and very standardized operation. In recent years, more and more attention has been paid to the construction of clinical gene amplification laboratory, because it plays a vital role in the reliability, accuracy and safety of detection results. This paper mainly expounds the main characteristics of the laboratory design from the plane layout, air conditioning and ventilation system design, air flow control and pollution prevention of the clinical gene amplification laboratory.
The core problem of clinical gene amplification laboratory design is how to avoid pollution. Therefore, the layout of the laboratory, the design of the air conditioning and ventilation system, and the air flow control are all carried out around this core issue. The following describes these aspects separately.