Publish Time: 2022-09-20 Origin: Site
(1) each experimental area is reasonably set;
(2) each experimental area shall be clearly marked (such as eye-catching door number or different ground color) to avoid confusion of equipment, articles, reagents, etc. in different experimental areas.
Reasonable system setting
(1) reasonable air conditioning and ventilation system shall be set, and the air conditioning system with full supply and discharge shall be adopted as far as possible;
(2) strict gas flow pressure control ensures different pressure requirements in different experimental areas.
Standard operation
(1) the technical personnel of clinical gene amplification testing laboratory must undergo on-the-job training, and can only engage in clinical gene amplification testing after passing the training;
(2) during the experimental operation, the operator must wear gloves and change them frequently. In addition, the use of disposable hats in operation is also an effective measure to prevent pollution;
(3) the cleaning work is timely and correct. After the experiment, the area must be cleaned immediately. In addition to wiping and disinfecting the surface with conventional disinfectant liquid or irradiating and disinfecting with ultraviolet lamps, some experimental equipment should also be disinfected under high pressure.
Strict management
(1) strictly control the personnel entering and leaving the laboratory. Personnel irrelevant to the experiment are not allowed to enter and exit the laboratory at will. If conditions permit, independent passages and doors to and from the whole experimental area should be set;
(2) work clothes with obvious distinguishing signs (such as different colors) shall be used in each experimental area. When the staff leave, they shall not take the work clothes in this area to other areas;
(3) minimize unnecessary walking in the experimental area to reduce the possibility of cross pollution.
(4) the amplification product analysis area is the main source of amplification product pollution. The waste liquid cannot be dumped in the laboratory. It must be soaked and disinfected with disinfectant and discarded away from the laboratory. Disposable materials such as used tips should also be soaked and disinfected with disinfectant before unified treatment, such as incineration;
(5) some mutagenic and toxic substances may be used in the amplification product analysis area, and special attention should be paid to the safety protection of laboratory personnel.
Complete laboratory supporting facilities
Complete laboratory supporting facilities are necessary to ensure the experimental work. Corresponding equipment and instruments should be equipped according to the different experimental contents of each laboratory, such as ultra clean workbench, centrifuge and sample dispenser.
There are no strict purification requirements for PCR laboratories, but in order to avoid the possibility of cross contamination among various experimental areas, it is appropriate to adopt the air distribution form of all sending and all discharging. At the same time, the proportion of air supply and exhaust should be strictly controlled to ensure the pressure requirements of each experimental area.
Reagent storage and preparation area
The main operations in this experimental area are the preparation of storage reagents, the sub packing of reagents and the preparation of the main reaction mixture. Reagents and materials used for specimen preparation shall be directly transported to this area and shall not pass through other areas. Reagent raw materials must be stored in this area and prepared into required storage reagents in this area.
There are no strict requirements for the control of air flow pressure in this area.