Views: 0 Author: Site Editor Publish Time: 2023-02-23 Origin: Site
The principle of laminar flow cabinet is based on the laminar flow of air through the cabinet.The device works by the use of inwards flow of air through one or more HEPA filters to create a particulate-free environment.The air is taken through a filtration system and then exhausted across the work surface as a part of the laminar flow of the air.The air first passes through the filter pad or pre-filter that allows a streamline flow of air into the cabinet.Next,the blower or fan directs the air towards the HEPA filters.The HEPA filters then trap the bacteria, fungi and other particulate materials so that the air moving out of it is particulate-free air.Some of the effluent air then passes through perforation present at the bottom rear end of the cabinet,but most of it passes over the working bench while coming out of the cabinet towards the face of the operator.The laminar flow hood is enclosed on the sides,and constant positive air pressure is maintained to prevent the intrusion of contaminated external air into the cabinet.
The procedure to be followed while operating a laminar flow cabinet is given below:
1.Before running the laminar flow cabinet,the cabinet should be checked to ensure that nothing susceptible to UV rays is present inside the cabinet.
2.The glass shield of the hood is then closed,and the UV light is switched on.The UV light should be kept on for about 15 minutes to ensure the surface sterilization of the working bench.
3.The UV light is then switched off,and a time period of around 10 minutes is spared before the airflow is switched on.
4.About 5 minutes before the operation begins the airflow is switched on.
5.The glass shield is then opened,and the fluorescent light is also switched on during the operation.
6.To ensure more protection the working bench of the cabinet can be sterilized with other disinfectants like 70% alcohol.
7.Once the work is completed,the airflow and florescent lamp both are closed and the glass shield is also closed.