Views: 0 Author: Site Editor Publish Time: 2022-09-22 Origin: Site
The main operations in this area are the preservation of clinical specimens, the extraction and storage of nucleic acids (RNA and DNA), and the synthesis of cDNA when it is added to the amplification reaction tube and RNA is measured.
The pressure gradient in this area is required to be positive pressure relative to the adjacent area to avoid aerosol pollution entering this area from the adjacent area. In addition, since aerosol pollution may occur during the sampling operation, unnecessary walking in the area should be avoided.
Amplification reaction mixture preparation and amplification area
The main operation in this region is DNA or cDNA amplification. In addition, the addition of the prepared DNA template and the synthesized cDNA (from the sample preparation area) and the preparation of the main reaction mixture (from the reagent storage and preparation area) into the reaction mixture can also be carried out in this area. In nested PCR determination, the reaction tube must be opened after the first round of amplification, so nested amplification has a high risk of contamination, and the second sampling must be carried out in this area.
The pressure gradient in this area is required to be negative pressure relative to the adjacent area to avoid aerosol leakage from this area. In order to avoid pollution caused by aerosols, unnecessary walking in the area should be minimized. Individual operations such as sample addition shall be carried out in the ultra clean bench.
Amplification product analysis area
The main operation in this area is the determination of amplified fragments. If full-automatic closed analytical instrument is used for detection, this area may not be set.
This area is the main source of amplification product pollution, so the pressure gradient in this area is required to be negative pressure relative to the adjacent area, so as to prevent amplification product from diffusing from this area to other areas.
Size of transfer window:
Specification of ordinary transfer window: sat500 500x500 (working size) sat600 600x600 (working size)
Specification of clean transfer window: sat600 600x600x600 (working size) sat1000 600x1000x600 (working size)