Polymerase Chain Reaction (PCR) is a fundamental technique in molecular biology, and ensuring a contamination-free environment is crucial for accurate results. The heart of this contamination control is the PCR cabinet. In this article, we will explore the essential factors to consider when selectin Read More

Depending on the direction of movement of air, laminar flow cabinets are divided into two types:1. Vertical laminar flow cabinet· In the vertical flow cabinets, the air moves from the top of the cabinet directly towards the bottom of the cabinet.· A vertical airflow working bench doe Read More

The principle of laminar flow cabinet is based on the laminar flow of air through the cabinet.· The device works by the use of inwards flow of air through one or more HEPA filters to create a particulate-free environment.· The air is taken through a filtration system and then exhaust Read More

A laminar flow hood/cabinet is an enclosed workstation used to create a contamination-free work environment by trapping all particles entering the cabinet through a filter.• • Designed to protect work from the environment, these cabinets are best suited for aseptic dispensing and plate dumping of sp Read More

PCR: Specimen preparation areaThe main operations in this area are the preservation of clinical specimens, the extraction and storage of nucleic acids (RNA and DNA). Read More

PCR: Strict division of work area(1) each experimental area is reasonably set;(2) each experimental area shall be clearly marked to avoid confusion of equipment, articles, reagents, etc. Read More

Plane layout of PCR LaboratoryIn principle, the clinical gene amplification laboratory is divided into four separate working areas: reagent storage and preparation area, sample preparation area, amplification reaction mixture preparation and amplification area. Read More

PCR work area and equipment requirements(I) reagent storage and preparation area1, 2-8c and - 15C refrigerators2. Mixer3. Micro sampler (covering 1-1000ul)4. Mobile UV lamp (near the workbench). Read More

PCR main equipmentThe instruments and equipment in the reagent preparation area mainly include refrigerator, super clean table, sample dispenser, mixer, balance and centrifuge. Read More