Publish Time: 2021-11-12 Origin: Site
Polymerase Chain Reaction (PCR) is a process where millions of copies of DNA are amplified from a single copy, or low copy number template. This reaction is fundamental to almost all applications requiring high copy number of starting material and is used in all laboratories working with DNA and RNA.
Because of the high copy number generated during PCR, it is essential to prevent possible contamination of the PCR reaction.
The ideal PCR laboratory should consist of three areas, each isolated from the other. Reagents should be prepared in the reagent preparation area and transferred to the sample preparation area, through a pass box, or inside closed containers. After preparation of the final reaction mix, the tubes should be transferred to the amplification area, again through a pass box or in a closed container. The PCR amplification and results analysis takes place in this area.
In practice, all these procedures are conducted in the same room. Under this circumstances, PCR Cabinets are used for reagent preparation and sample preparation to minimize contamination. In the case of biohazardous samples, biological safety cabinets must be used for sample preparation.
Ozone free high density UV decontamination.
Long living UV lamps (8,000 hours average).
Automatic switch off of UV-lamps when the protective screen is opened. Bactericidal flow-type recirculator providing permanent decontamination inside UV –cabinet during operation.
Shockproof glass walls Low noise, low energy consumption.
Powerful, uniformly distributed, UV-C.
Easy to use
• Timer is easy to adjust.
• UV hour meter monitors bulb life.
• Automatic decontamination for 0.9 m (3') and 1.2 m (4') models - Close sash: UV turns on automatically for decontamination. - Open sash: airflow activates automatically.
Safety
• UV interlock prevents UV exposure.
• UL recognized electrical components.
• UV filtering sash and side walls.
Ergonomics
• Low noise.
• Angled front.
• Glass sides.
• Curved work surface front edge.
• Powder coated rear wall eliminates reflections.
• Vertical air flow minimizes direct airflow, dry eyes.
It is bench-top type, made of metal framework, glass (or plexiglas) walls and working surface painted with powder enamel or made of stainless steel. PCR cabinets are equipped with an open UV lamp installed in the upper hood. UV-radiation from the open lamps disinfects the working area inactivating DNA/RNA fragments during 15–30 min of exposure. A digital timer controls duration of the direct UV irradiation. A daylight lamp provides proper illumination of the working surface.
PCR cabinets are equipped with a flow-type bactericidal UV cleaner–recirculator AR, which provides constant decontamination inside the cabinet during operation. They are recommended for operations with DNA/RNA amplicons. UV cleaner–recirculator AR consists of a UV lamp, a fan and dust filters organized in a special body so that a user working with a PCR cabinet is protected against UV light. Recirculator increases the maximum density of UV light making it sufficiently effective for DNA/RNA inactivation. The UV–recirculator processes 100 PCR cabinet volumes per hour, creating permanent aseptic conditions of operation inside the PCR cabinet.