Publish Time: 2022-02-02 Origin: Site
An clean bench is a facility that provides a sterile operating environment for laboratory work to protect the experiment from the external environment, while providing some degree of protection for the external environment to prevent contamination and protect the operator. With a simple sterile hood In contrast, the clean bench has the advantages of allowing the operator to move freely, easily reaching any place in the operating area, and having high integrity.
The clean environment of the ultra-clean workbench is formed by the flow of clean air (filtered air) in a set direction in a specific space. According to the direction of the airflow, the existing ultra-clean workbenches can be divided into vertical type, From the inside to the outside and sideways. Considering the quality of operation and the impact on the environment, the vertical type is superior. The clean air provided by the air supply filter plate descends through the operation area at a specific speed. It is separated in the middle and sucked away by the front air suction hole and the rear suction window. The air sucked in the lower part of the operation area and the front part are mixed together and pumped into the rear positive pressure area by the blower. In the upper part of the machine, 30% of the gas passes through the exhaust The air filter panel is exhausted from the top, and about 70% of the gas is re-entered into the operating area through the oxygen supply filter panel. To supplement the air discharged from the exhaust port, the same volume of air is supplemented from the room air through the operating port. These air will never Entering the operating area, it just forms an air barrier.
But this does not mean that the manufacturer's test can replace the operator's necessary inspection and debugging before use. Because the environment of the ultra-clean workbench is different, only after proper inspection and debugging can the equipment function to the maximum extent. A better environment should be selected for the machine before commissioning. It is best to place it in a sterile room with air disinfection facilities. If the conditions are not available, the machine should be placed in a place with less movement and cleaner Adjust the height of each foot to ensure the stability and the level of the operating surface. The power supply of the ultra-clean workbench should use a special circuit to avoid the change of the air flow rate caused by the overload of the circuit. Compared with the simple sterile hood, The ultra-clean bench has the ability to allow the operator to move freely, easily reach any place in the operating area, and an ultraviolet germicidal lamp and fluorescent lamp for lighting are standard configurations of the ultra-clean bench. The blower provides the power for air flow, and it is clear at a glance whether these components are working properly. .The most difficult and most important is to check the air filter plate and its tightness, which is directly related to the normal use of the machine. The easiest way to check is the nutrient agar plate method. The newly purchased and unused ultra-clean workbenches are removed. When the machine is in working state, place an open nutrient agar plate at each of the four corners and the center of the operation area, cover it after two hours, and place it in a 37°C incubator. Incubate in medium for 24 hours, and calculate the number of colonies. The average number of colonies per plate must be less than 0.5.
Before using the ultra-clean workbench, turn on the ultraviolet light for 30-40 minutes, and check that the various openable doors and windows around the operation area are in the working position. The operation is best done in the center of the operation area. A safer area. Before operating, you should have a preliminary understanding of the experimental materials, and at the same time understand the performance and safety level of the equipment you use. Strictly implement laboratory safety regulations. The use of specific pathogens in any ultra-clean bench must be Carry out safety assessment. If the experimental material will cause environmental pollution to the surrounding environment, it should be avoided in the model without exhaust filter plate, because operation in flowing air is no different from scattered poison. Any advanced equipment does not guarantee the experiment To be successful, the use of ultra-clean workbenches in animal quarantine laboratories is aimed at sterility and avoiding cross-contamination, so skilled operation and clear aseptic essentials are essential.
Maintenance The ultra-clean workbench is a relatively precise electrical equipment, and it is very important to carry out regular maintenance and maintenance. First of all, it is necessary to keep the room dry and clean. The humid air will not only corrode the manufacturing materials, but also affect the The normal operation of the electrical circuit, moist air is also conducive to the growth of bacteria and mold. A clean environment can also prolong the service life of the filter plate. In addition, regular cleaning of the equipment is an important part of normal use. Cleaning should include routine before and after use Cleaning and regular treatment. When fumigating, all gaps should be completely sealed. For example, the operation port is equipped with a type of ultra-clean workbench with a movable baffle cover, which can be sealed with plastic film. The filter plate and ultraviolet germicidal lamp of the ultra-clean workbench All have a specified service life and should be replaced on time.